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Contributor Information

  • Name David Garrod
  • Institute University of Southampton

Tool Details

  • Tool name: Anti-Desmoglein 1 [32-2B]
  • Alternate names: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1
  • Clone: 32-2B
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Bovine ; Human
  • Host: Mouse
  • Application: EM (EM) ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.
  • Immunogen: Bovine desmoglein (desmosomal glycoprotein I) from nasal epidermis
  • Isotype: IgG2a kappa
  • Research area: Cell biology; Cancer; Cell signaling and signal transduction
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Desmoglein 1
  • Target background: Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.

Application Details

  • Application: EM (EM) ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Maruani et al. 2008. Am J Clin Pathol. 130(3):369-74. PMID: 18701409.
  • •  Immunostaining with antibodies to desmoglein provides the diagnosis of drug-induced pemphigus and allows prediction of outcome.
  • •  A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
  • •  Vilela et al. 1995. J Cell Sci. 108 ( Pt 4):1743-50. PMID: 7615689.
  • •  Harada et al. 1992. Int J Oral Maxillofac Surg. 21(6):346-9. PMID: 1282923.
  • •  Immunohistochemical detection of desmosomes in oral squamous cell carcinomas: correlation with differentiation, mode of invasion, and metastatic potential.
  • •  Conn et al. 1990. Br J Urol. 65(2):176-80. PMID: 2317652.
  • •  Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas.
  • •  Vilela et al. 1987. J Pathol. 153(4):365-75. PMID: 3323436.
  • •  Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.