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Contributor Information

  • Name Al Charest
  • Institute Tufts University

Tool Details

  • Tool name: Anti-cROS [4-6G]
  • Clone: 4-6G
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF ; IP ; Fn ; WB
  • Description: Monoclonal antibody directed against cROS, a proto-oncogene with therapeutic target potential.
  • Immunogen: Extracellular portion of ROS amino acid 1-285 fused to Fc, transiently expressed in 293 cells and purified using PtnA column chromatography.
  • Immunogen UniProt ID: P08922
  • Isotype: IgG1
  • Research area: Cancer; Cell signaling and signal transduction; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: cROS
  • Target background: c-ROS is a proto-oncogene tyrosine kinase, that is highly-expressed in a variety of tumour cell lines and belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. It is a type I integral membrane protein and may function as a growth or differentiation factor receptor. The c-ROS gene promoter region has been identified and characterized and it has been shown that the ectopic expression of c-ROS in tumours is tied to hypomethylation of a CpG island in the c-ROS promoter. Tumours with ROS1 mutations are typically responsive to small molecule tyrosine kinase inhibitors.

Application Details

  • Application: IHC ; IF ; IP ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Fei et al. 2019. Biosci Rep. 39(6):. PMID: 31138757.
  •   Zhang et al. 2019. Int J Biol Sci. 15(7):1460-1471. PMID: 31337976.
  •   Shih et al. 2017. Oncogene. 36(47):6542-6554. PMID: 28759046.
  •   Deng et al. 2014. Int J Mol Med. 34(3):661-8. PMID: 24968753.
  •   Charest et al. 2003. Proc Natl Acad Sci U S A. 100(3):916-21. PMID: 12538861.
  •   Oncogenic targeting of an activated tyrosine kinase to the Golgi apparatus in a glioblastoma.