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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-WIPI2 [2A2]
  • Clone: 2A2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Molecular weight of the target: 49 kDa
  • Application: IF ; FACS ; IF ; IP ; WB
  • Description: This antibody is useful for the detection of WIPI-2, an essential protein for autophagy.
  • Immunogen: Synthetic peptide corresponding to C-terminus of WIPI2b CSALRLDEDSEHPPMILRTD
  • Isotype: IgG1 kappa
  • Research area: Cancer; Tissue-specific biology; Biochemistry

  • For Research Use Only

Target Details

  • Target: WIPI2
  • Target molecular weight: 49 kDa
  • Target background: WIPI2 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. WIPI2 is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. WIPI-2 is the mammalian homologue of Atg18.

Application Details

  • Application: IF ; FACS ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Nishimura et al. 2017. EMBO J. 36(12):1719-1735. PMID: 28495679.
  • •  Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.
  • •  Martinez-Martin et al. 2017. Science. 355(6325):641-647. PMID: 28183981.
  • •  A switch from canonical to noncanonical autophagy shapes B cell responses.
  • •  Polson et al. 2010. Autophagy. 6(4):506-22. PMID: 20505359.
  • •  Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
  • •  Li et al. 2005. J Biol Chem. 280(29):26941-52. PMID: 15917248.
  • •  Krppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1.
  • •  Benzeno et al. 2004. Cancer Res. 64(11):3885-91. PMID: 15172998.
  • •  Cyclin-dependent kinase inhibition by the KLF6 tumor suppressor protein through interaction with cyclin D1.