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Contributor Information

  • Name Paul Crocker
  • Institute University of Dundee

Tool Details

  • Tool name: Anti-SIGLEC5 [1A5]
  • Alternate names: Sialic Acid Binding Ig Like Lectin 5; Obesity-Binding Protein 2; OB-Binding Protein 2; CD33 Antigen-Like 2; SIGLEC-5; CD33L2; OBBP2; Sialic Acid-Binding Immunoglobulin-Like Lectin 5; CD17 Antigen;
  • Clone: 1A5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; ELISA ; FACS ; IHC ; IF ; IP ; Fn ; WB
  • Description: The Siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Siglec5 (CD170) is a member of the recently-described human CD33-related siglec subgroup of sialic acid binding Ig-like lectins and is expressed on myeloid cells of the hemopoietic system. Similar to other CD33- related siglecs, Siglec-5 contains two tyrosine based motifs in its cytoplasmic tails implicated in signalling functions. The antibody cross reacts with Siglec14.
  • Immunogen: Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1
  • Isotype: IgG1
  • Research area: Cell biology; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Siglec5
  • Target background: The Siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Siglec5 (CD170) is a member of the recently-described human CD33-related siglec subgroup of sialic acid binding Ig-like lectins and is expressed on myeloid cells of the hemopoietic system. Similar to other CD33- related siglecs, Siglec-5 contains two tyrosine based motifs in its cytoplasmic tails implicated in signalling functions. The antibody cross reacts with Siglec14.

Application Details

  • Application: IHC ; ELISA ; FACS ; IHC ; IF ; IP ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -80°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.
  • •  Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
  • •  Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.
  • •  Loss of Siglec expression on T lymphocytes during human evolution.
  • •  Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.
  • •  Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.
  • •  Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929
  • •  Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.
  • •  Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.
  • •  Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.