ANTIBODIES

Contributor Information
- Name Alison Goodall
- Institute University College London (UCL)
Tool Details
- Tool name: Anti-von Willebrand Factor [RFF-VIII:R/2]
- Clone: RFF-VIII:R/2
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Primate
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB
- Description: The monoclonal antibody RFF-VIII:R/1 specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/1 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg. This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits it's binding to platelets. Very similar in characteristics to RFF-VIII:R/2
- Immunogen: Purified human Factor VIII-VWF complex
- Isotype: IgG1 kappa
- Research area: Tissue-specific biology; Stem cell biology
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: Von Willebrand factor (VWF)
- Target background: The monoclonal antibody RFF-VIII:R/1 specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/1 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg. This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits it's binding to platelets. Very similar in characteristics to RFF-VIII:R/2
Application Details
- Application: ELISA ; FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB
Handling
- Format: Liquid
- Shipping conditions: Shipping at 4ðC
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References
- • [Use of RFF-VIII:R/1 in the purification of FVIII]
- • Murdock et al. 1997. Thromb Haemost. 78(4):1272-7. PMID: 9364997.
- • von Willebrand factor activity detected in a monoclonal antibody-based ELISA: an alternative to the ristocetin cofactor platelet agglutination assay for diagnostic use.
- • Takase et al. 1987. Br J Haematol. 66(4):497-502. PMID: 3117089.
- • Production of factor VIII deficient plasma by immunodepletion using three monoclonal antibodies.
- • McCraw et al. 1987. Thromb Res. 45(1):101-8. PMID: 3494325.
- • A monoclonal antibody based immunoradiometric assay for von Willebrand factor: survey of a large patient group.
- • Chand et al. 1986. Thromb Haemost. 55(3):318-24. PMID: 2428124.
- • A two-site, monoclonal antibody-based immunoassay for von Willebrand factor--demonstration that vWF function resides in a conformational epitope.
- • Rotblat et al. 1985. Biochemistry. 24(16):4294-300. PMID: 2413885.
- • Purification of human factor VIII:C and its characterization by WBting using monoclonal antibodies.
- • Goodall et al. 1985. Br J Haematol. 59(4):565-77. PMID: 2580547.
- • An immunoradiometric assay for human factor VIII/von Willebrand factor (VIII:vWF) using a monoclonal antibody that defines a Fn epitope.