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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-XPF [XPF 3F2/3]
  • Clone: XPF 3F2/3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. ERCC1 and XPF form the heterodimer ERCC1-XPF, forming a DNA endonuclease that is essential for the dual incision step of NER (cleaves 5' of the DNA lesion).
  • Immunogen: A recombinant His-tagged fragment of human XPF protein produced in E. coli
  • Isotype: IgG1
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: XPF
  • Target background: Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. ERCC1 and XPF form the heterodimer ERCC1-XPF, forming a DNA endonuclease that is essential for the dual incision step of NER (cleaves 5' of the DNA lesion).

Application Details

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Facista et al. 2012. Genome Integr. 3(1):3. PMID: 22494821.
  • •  Deficient expression of DNA repair enzymes in early progression to sporadic colon cancer.
  • •  Bhagwat et al. 2009. Cancer Res. 69(17):6831-8. PMID: 19723666.
  • •  Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.