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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Joy Burchell
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-MUC1 [HMFG1]
  • Alternate names: ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
  • Clone: HMFG1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Breast cancer; Gynaecologic cancer; Digestive / Gastrointestinal cancer;
  • Application: FACS ; ELISA ; FACS ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Mucin-1 (MUC1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MUC1 is also localised on the surface of the human milk fat globule. MUC1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. It is upregulated in the lactating breast and in carcinomas.
  • Immunogen: Delipidated human milk fat globule.
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Mucin1 (MUC1)
  • Target background: Mucin-1 (MUC1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MUC1 is also localised on the surface of the human milk fat globule. MUC1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. It is upregulated in the lactating breast and in carcinomas.

Application Details

  • Application: FACS ; ELISA ; FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.
  • •  Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.
  • •  Moss et al. 1988. Lung Cancer. 4, 76-78.
  • •  Burchell et al. 1987. Cancer Res. 47(20):5476-82. PMID: 2443241.
  • •  Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin.
  • •  Chang et al. 1986. Biochim Biophys Acta. 823(3):161-94. PMID: 2423124.
  • •  In vitro transformation of human epithelial cells.
  • •  Burchell et al. 1983. J Immunol. 131(1):508-13. PMID: 6190927.
  • •  Complexity of expression of antigenic determinants, recognized by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells.
  • •  Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.
  • •  Arklie et al. 1981. Int J Cancer. 28(1):23-9. PMID: 6273328.
  • •  Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers.
  • •  Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.