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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-HSP27 [ACD5]
  • Alternate names: Heat Shock Protein Family B (Small) Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein; HSP27; HSP28; SRP27; Epididymis Secretory Protein Li 12; HEL-S-12; HS.7667; CMT2F; HMN2B; Hsp25; HspB1
  • Clone: ACD5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 27 kDa
  • Application: IHC ; IP
  • Strain: Balb/c
  • Description: The anti estrogen receptor (RE) antibody ACD5 (also known as D5) is capable of precipitating labelled human cytosolic RE complexes from breast tumour, fibroid, myometrial and endometrial preparations, but does not react with nuclear RE from human endometrium or cytoplasmic RE from other species tested. The antibody does not react with progesterone receptor or androgen receptor from human breast tissue, sex steroid binding globulin (SHBG) from human plasma, or rat alpha fetoprotein. The antibody is associated with a 29KDa component in RE-positive cytosols, in addition the antigen is a non hormone binding component related to cytosolic RE from breast tumour and myometrium.
  • Immunogen: Purified cytoplasmic oestrogen receptor fragment from human myometrium.
  • Isotype: IgG1
  • Research area: Cancer; Tissue-specific biology; Cell signaling and signal transduction
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: HSP27
  • Target molecular weight: 27 kDa

Application Details

  • Application: IHC ; IP

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Zhu et al. 1994. J Biol Chem. 269(35):22379-84. PMID: 7915282.
  • •  Specific binding of the transglutaminase, platelet factor XIII, to HSP27.
  • •  Love et al. 1994. Br J Cancer. 69(4):743-8. PMID: 8142264.
  • •  A 27 kDa heat shock protein that has anomalous prognostic powers in early and advanced breast cancer.
  • •  Dunn et al. 1993. J Steroid Biochem Mol Biol. 46(4):469-79. PMID: 8217877.
  • •  Relationship of HSP27 and oestrogen receptor in hormone sensitive and insensitive cell lines.
  • •  Coffer et al. 1985. Cancer Res. 45(8):3686-93. PMID: 4016746.
  • •  Monoclonal antibodies against a component related to soluble estrogen receptor.