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Contributor Information

  • Name Roy Jefferis
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-LAT1 [BU53]
  • Alternate names: Solute Carrier Family 7 Member 5; L-Type Amino Acid Transporter; Integral Membrane Protein E16; CD98 Light Chain; 4F2 Light Chain; MPE16; HLAT1; LAT1; Sodium-Independent Neutral Amino Acid Transporter LAT1; Large Neutral Amino Acids Transporter 1; D16S469E; CD98LC; CD98; E16
  • Clone: BU53
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC
  • Description: Monoclonal antibody directed against LAT1 transporter channel protein and can aid investigation into amino acid transporter channel upregulation, a common mutation in oncogenesis.
  • Immunogen: Plasma cell line 8226
  • Immunogen UniProt ID: Q01650
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell signaling and signal transduction; Metabolism

  • For Research Use Only

Target Details

  • Target: Large neutral Amino acid Transporter 1 (LAT1, CD98)
  • Target background: LAT1, also known as SLC7A5, is part of the APC superfamily and forms a heterodimeric amino acid transporter channel. LAT1 interacts with CD98 glycoprotein via a conserved disulphide. This transporter channel is involved in the uptake of essential amino acids in various regions, particularly the placenta and blood brain barrier. LAT1 expressed on numerous cell types including B cells, activated T cells, monocytes, granulocytes, erythrocytes and endothelial cells. LAT1 is up-regulated on leucocytes in inflammatory lesions and is strongly expressed by neoplastic cells. Changes in amino acid transporter expression, including LAT1, are thought to be an early change in oncogenesis, as they provide growth and survival advantages.

Application Details

  • Application: FACS ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Cho et al. 2001. Blood. 98(2):374-82. PMID: 11435306.
  • •  The Fn interactions between CD98, beta1-integrins, and CD147 in the induction of U937 homotypic aggregation.
  • •  Leukocyte Typing VI 1997 Garland Publishing.