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Contributor Information

  • Name David Lane
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
  • Primary citation Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

Tool Details

  • Tool name: Anti-p53 [Pab 246]
  • Clone: Pab 246
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 50 kDa
  • Application: IHC ; IP
  • Strain: Balb/c
  • Description: Anti-p53 clone Pab 246 recognises the tumour antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is upregulated in response to DNA damage and is found in a wide variety of transformed cells. Mutations in p53 can lead to conformational changes in the protein, exposing different epitopes. Pab 246 binds to the wild-type conformation of p53
  • Immunogen: SV40-transformed cell line Balb/c SVA31 E7.
  • Immunogen UniProt ID: P02340
  • Isotype: IgG1 kappa
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: p53 mouse, wild type only
  • Target molecular weight: 50 kDa
  • Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.

    Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy.

    Twelve isoforms of p53 have been described. Depending on the location of the epitope, antibodies may detect different isoforms of p53. Mutations to p53 can cause conformational changes that expose different epitopes. Pab 246 binds to the wild-type conformation of p53.

Application Details

  • Application: IHC ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Pradhan et al. 2019. Nucleic Acids Res. 47(4):1637-1652. PMID: 30649466.
  • •  Hwang et al. 2018. Cell Rep. 22(1):299-312. PMID: 29298430.
  • •  Labalette et al. 2008. PLoS One. 3(11):e3761. PMID: 19018287.
  • •  Han et al. 2008. Cell Stem Cell. 2(3):241-51. PMID: 18371449.
  • •  Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.
  • •  Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.
  • •  Wade-Evans et al. 1985. EMBO J. 4(3):699-706. PMID: 2408882.