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Contributor Information

  • Name Jacqueline Cordell
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-EMA [E29] mAb
  • Clone: E29
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; WB
  • Description: Monoclonal antibody used as a pan-epithelial marker to categorise cancers of epithelial origin and detect metastatic loci.
  • Immunogen: Human milk fat globule membrane preparation
  • Immunogen UniProt ID: P15941
  • Isotype: IgG2a
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Epithelial Membrane Antigen (EMA)
  • Target background: Glycoproteins isolated from human milk fat globule membranes, designated epithelial membrane antigen (EMA), have been detected immunohistochemically in most non-neoplastic epithelia and are potentially a highly effective marker for establishing the epithelial nature of neoplastic cells. Anti-EMA was created for use in diagnostic immunocytochemistry for identification of tumours of epithelial origins. EMA is present within membranes of the apical side of secretory epithelia, and is typically overexpressed in colon, breast, ovarian, lung and pancreatic cancers.

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

Documentation

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References

  • •  A case of a CD56-expressing ectomesenchymal chondromyxoid tumor of the tongue: potential diagnostic usefulness of commonly available CD56 over CD57
  • •  Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?
  • •  Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.
  • •  Maxillary carcinosarcoma: Identification of a novel MET mutation in both carcinomatous and sarcomatous components through next generation sequencing.
  • •  Liu et al. 2015. Breast Cancer Res Treat. 151(2):443-51. PMID: 25953688.
  • •  Habougit et al. 2015. Int J Surg Pathol. :. PMID: 26113666.
  • •  Mediastinal Mature Teratoma With Malignant Carcinomatous Transformation (Somatic-Type Malignancy) With Metastatic Course.
  • •  Kim et al. 2015. PLoS One. 10(3):e0120296. PMID: 25798919.
  • •  Invasive micropapillary mucinous carcinoma of the breast is associated with poor prognosis.
  • •  Ohlmann et al. 2015. Ann Diagn Pathol. :. PMID: 25990776.
  • •  Sclerosing epithelioid fibrosarcoma of the kidney: clinicopathologic and molecular study of a rare neoplasm at a novel location.
  • •  Genome-wide transcript profiling reveals novel breast cancer-associated intronic sense RNAs.
  • •  Ando et al. 2015. Head Neck. :. PMID: 25782598.
  • •  ten Berge et al. 2001. J Clin Pathol. 54(12):933-9. PMID: 11729213.
  • •  MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.
  • •  Heyderman et al. 1985. Br J Cancer. 52(3):355-61. PMID: 3899156.
  • •  Cordell et al. 1985. Br J Cancer. 52(3):347-54. PMID: 3899155.
  • •  A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
  • •  Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.