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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-ASF1 [ASF 4A1/3]
  • Clone: ASF 4A1/3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Application: WB
  • Strain: Balb/c
  • Description: Anti-silencing function 1 (Asf1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled-like kinase (TLK). The Tousled kinases are themselves regulated by the checkpoint protein kinases involved in the DNA damage response.
  • Immunogen: FLAG-ASF1A
  • Isotype: IgG2a
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Anti-Silencing Function 1 (ASF1)
  • Target background: Anti-silencing function 1 (Asf1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled-like kinase (TLK). The Tousled kinases are themselves regulated by the checkpoint protein kinases involved in the DNA damage response.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Sillj et al. 2001. Curr Biol. 11(13):1068-73. PMID: 11470414.
  • •  Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases.