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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-CyclinA [E67.1]
  • Alternate names: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2 Human Entrez Gene ID: 89 Human SwissProt: P2248 Human Unigene: 58974 Human Gene Symbol: CCNA2 Human Chromosome Location: 4q27
  • Clone: E67.1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Bovine ; Human ; Mink ; Mouse
  • Host: Mouse
  • Molecular weight of the target: 54 kDa
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase.
  • Immunogen: Full length bovine Cyclin A protein
  • Immunogen UniProt ID: P78396
  • Isotype: IgG2a kappa
  • Research area: Cancer; Cell biology; Genetics

  • For Research Use Only

Target Details

  • Target: Cyclin A
  • Target molecular weight: 54 kDa
  • Target background: The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204.
  • •  Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
  • •  Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
  • •  Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
  • •  Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
  • •  Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
  • •  Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
  • •  Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
  • •  Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
  • •  Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
  • •  Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
  • •  Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
  • •  A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
  • •  Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
  • •  Simian virus 40 large T antigen associates with cyclin A and p33cdk2.