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Contributor Information

  • Name David Lane
  • Institute University of Dundee

Tool Details

  • Tool name: Anti-MDM2 [SMP14]
  • Clone: SMP 14
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
  • Immunogen: Synthetic peptide CSRPSTSSRRRAISE (residues 154-167 in human MDM2) cross-linked to KLH using glutaldehyde.
  • Isotype: Ig3 Kappa
  • Research area: Cell biology; Cancer; Genetics
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Murine Double Minute 2 protein (MDM2)
  • Target background: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.

Application Details

  • Application: ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.
  • •  Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.
  • •  Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
  • •  Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
  • •  Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.
  • •  p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.
  • •  Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.
  • •  A melanoma-predisposing germline CDKN2A mutation with Fn significance for both p16 and p14ARF.
  • •  Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.
  • •  Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
  • •  Bttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.
  • •  Molecular characterization of the hdm2-p53 interaction.
  • •  Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.
  • •  Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.
  • •  Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.
  • •  Immunochemical analysis of the interaction of p53 with MDM2
  • •  --fine mapping of the MDM2 binding site on p53 using synthetic peptides.