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Contributor Information

  • Name Stephen West
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Rad51D [Rad51D 5B3/6]
  • Clone: Rad51D 5B3/6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IP ; WB
  • Strain: Balb/c
  • Description: RAD51D is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51D is also a component for the homologous recombination pathway.
  • Immunogen: His-tagged human Rad51D, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page
  • Isotype: IgG1
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Rad51D
  • Target background: RAD51D is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51D is also a component for the homologous recombination pathway.

Application Details

  • Application: IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
  • •  Identification and purification of two distinct complexes containing the five RAD51 paralogs.
  • •  Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.
  • •  Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family.
  • •  Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.
  • •  Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene.