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Contributor Information

  • Name Stephen West
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Rad51B [rad51b 1h3/13]
  • Clone: rad51b 1h3/13
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IP ; WB
  • Strain: Balb/c
  • Description: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
  • Immunogen: Rad51b 1h3/13 was raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-PAGE.
  • Isotype: IgG2a
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Rad51B
  • Target background: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.

Application Details

  • Application: IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Chun et al. 2013. Mol Cell Biol. 33(2):387-95. PMID: 23149936.
  • •  Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
  • •  Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
  • •  Identification and purification of two distinct complexes containing the five RAD51 paralogs.
  • •  Albala et al. 1997. Genomics. 46(3):476-9. PMID: 9441753.
  • •  Identification of a novel human RAD51 homolog, RAD51B.