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Contributor Information

  • Name John Marshall
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Integrin a4 [7.2]
  • Clone: 7.2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IP ; WB
  • Description: Monoclonal antibody directed at integrin a4/b1, which forms part of VLA-4.
  • Immunogen: Human melanoma line DX3
  • Immunogen UniProt ID: P13612
  • Isotype: IgG1
  • Research area: Cell biology; Tissue-specific biology; Cell signaling and signal transduction; Immunology; Stem cell biology

  • For Research Use Only

Target Details

  • Target: Integrin alpha 4 (VLA-4; CD49d)
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha 4 combines with beta-1 integrin to form the Integrin alpha4/ beta1 lymphocyte homing receptor: Very Late Antigen 4 (VLA-4). VLA-4 plays a major role in lymphocyte extravasation, via binding to VCAM on endothelial cells. VLA-4 also binds fibronectin and mediates homotypic and heterotypic cell-cell adhesion between lymphocytes. Integrin alpha 4/ beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Application Details

  • Application: FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  McDowall et al. 2003. J Clin Invest. 111(1):51-60. PMID: 12511588.
  • •  A novel form of integrin dysfunction involving beta1, beta2, and beta3 integrins.
  • •  Leitinger et al. 2002. J Cell Sci. 115(Pt 5):963-72. PMID: 11870215.
  • •  The involvement of lipid rafts in the regulation of integrin function.