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Contributor Information

  • Name Helen Turley
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-MUC18 [OJ79c MUC 18]
  • Alternate names: ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
  • Clone: OJ79c MUC 18
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 130 kDa
  • Application: FACS ; IHC ; IF
  • Description: Monoclonal antibody directed against a marker of melanoma and metastasis.
  • Immunogen: Recombinant MUC18 (D1-D5) Fc chimeric protein
  • Immunogen UniProt ID: P43121
  • Isotype: IgG1 kappa
  • Research area: Cell biology; Cancer; Tissue-specific biology; Cell signaling and signal transduction; Immunology; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: MUC18 (CD146)
  • Target molecular weight: 130 kDa
  • Target background: CD146 has been identified as a cell adhesion molecule and is useful in differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 is expressed at cell-cell junctions in all endothelial cells. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogenous tumour spread. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease. It is associated with poor prognosis.

Application Details

  • Application: FACS ; IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Smith et al. 2011. Immunology. 132(2):256-65. PMID: 21039468.
  • •  Sato et al. 2010. Mod Pathol. 23(11):1458-66. PMID: 20657552.
  • •  Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
  • •  Iohara et al. 2008. Stem Cells. 26(9):2408-18. PMID: 18583536.
  • •  Crisan et al. 2008. Cell Stem Cell. 3(3):301-13. PMID: 18786417.
  • •  A perivascular origin for mesenchymal stem cells in multiple human organs.
  • •  A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
  • •  Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
  • •  Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
  • •  Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.
  • •  Kuzu et al. 1993. Lab Invest. 69(3):322-8. PMID: 7690867.
  • •  Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.