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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Cig2 [CIG2 3A11/5]
  • Alternate names: Cyc17
  • Clone: CIG2 3A11/5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Schizosaccharomyces pombe
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; IP ; WB
  • Description: Cig2 is a fission yeast (S. pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (Cdk1), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
  • Immunogen: S. pombe Cig2 purified protein (bacterial expression)
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Cig2
  • Target background: Cig2 is a fission yeast (S. pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (Cdk1), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.

Application Details

  • Application: ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Chu et al. 2009. Mol Cell Biol. 29(18):4959-70. PMID: 19596787.
  • •  A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast.
  • •  Chu et al. 2007. Mol Biol Cell. 18(5):1756-67. PMID: 17332498.
  • •  Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast.
  • •  Yamano et al. 2000. Mol Cell. 6(6):1377-87. PMID: 11163211.
  • •  The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.