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Contributor Information

  • Name John Kemshead
  • Institute Institute of Child Health

Tool Details

  • Tool name: Anti-L1CAM [UJ127.11]
  • Alternate names: CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S1, SPG1
  • Clone: UJ127.11
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Molecular weight of the target: 220-240 kDa
  • Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody capable of differentiating between brain tumours of neural origin rather than glial origin.
  • Immunogen: Homogenous suspension of 16 week human foetal brain.
  • Immunogen UniProt ID: P32004
  • Isotype: IgG1 kappa
  • Research area: Cell biology; Cell signaling and signal transduction; Neurobiology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: L1 cell adhesion molecule (L1CAM; CD171)
  • Target molecular weight: 220-240 kDa
  • Target background: Anti-L1CAM (UJ127.11) recognises L1 cell adhesion molecule (L1CAM), a cell-surface glycoprotein of 220-240kDa (doublet) and member of the neural adhesion molecule family of the immunoglobulin superfamily. UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma) or for the purpose of bone marrow purging. Studies on normal foetal and adult tissues show that UJ127:11 recognises antigen restricted in its expression to cells of neural rather than glial origin. Neural tumours such as neuroblastoma, medulloblastoma, schwannomas and ganglioglioma bind the monoclonal antibody whereas malignancies originating from glial cells do not bind UJ 127:11 this allows for diagnosis of the origin of brain tumour. This antibody has little cross-reactivity with tissues outside of the neuroectoderm. L1CAM is implicated in the development of the nervous system and its expression is restricted to tissues arising from the neuroectoderm. L1CAM is present on tumours of neuroectodermal and glial origin (e.g. neuroblastoma and schwannomas). L1CAM also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Application Details

  • Application: ELISA ; FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Maten et al. 2019. Int J Mol Sci. 20(17):. PMID: 31455004.
  • •  Pace et al. 2019. Int J Mol Sci. 20(16):. PMID: 31426278.
  • •  Stevers et al. 2019. Mod Pathol. 32(1):88-99. PMID: 30171198.
  • •  Wang et al. 2018. Neurobiol Dis. 116:53-59. PMID: 29705185.
  • •  Er et al. 2018. Nat Cell Biol. 20(8):966-978. PMID: 30038252.
  • •  Zaatiti et al. 2018. Int J Oncol. 52(3):787-803. PMID: 29328367.
  • •  Wood et al. 2018. Brain Pathol. 28(2):192-202. PMID: 28960623.
  • •  Fontanals-Cirera et al. 2017. Mol Cell. 68(4):731-744.e9. PMID: 29149598.
  • •  Agrawal et al. 2017. Cancer Cell. 31(6):804-819.e7. PMID: 28609658.
  • •  Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.
  • •  Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.
  • •  Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.
  • •  Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.
  • •  Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.
  • •  Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.
  • •  Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.
  • •  Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.
  • •  Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.
  • •  Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.
  • •  Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.
  • •  Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.