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Contributor Information

  • Name Peter Beverley
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CD45RO [UCH-L1]
  • Alternate names: Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T2; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T2 Leukocyte Common Antigen; T2 Glycoprotein; EC 3.1.3.48; GP18; CD45R; B22; LCA; LY5
  • Clone: UCH-L1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 180 kDa
  • Application: FACS ; IHC ; IP ; WB
  • Strain: Balb/c
  • Description: Highly specific monoclonal antibody which can detect CD45RO, differentiating between subpopulations of T lymphocytes.
  • Immunogen: Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
  • Immunogen UniProt ID: P08575
  • Isotype: IgG2a
  • Research area: Cancer; Cell biology; Tags and cell markers; Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO)
  • Target molecular weight: 180 kDa
  • Target background: CD45RO (UCHL1) is also known as protein tyrosine phosphatase receptor type C (PTPRC) or leukocyte common antigen. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling.

Application Details

  • Application: FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Elgueta et al. 2019. Front Immunol. 10:981. PMID: 31118938.
  • •  Moura et al. 2017. Cell Mol Immunol. 14(9):758-769. PMID: 26996067.
  • •  Wu et al. 2015. Oncotarget. 6(30):28633-45. PMID: 26416419.
  • •  Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.
  • •  Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.
  • •  Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
  • •  Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
  • •  Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.
  • •  CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.
  • •  Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.
  • •  Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.
  • •  Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.
  • •  Fn subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.