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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, Lincoln's Inn Fields Institute
  • Primary citation Hudson et al. 1992. Hybridoma. 11(3):367-379. PMID: 1500072.

Tool Details

  • Tool name: Anti-Involucrin [SY5] mAb
  • Alternate names: Anti-Involucrin; involucrin antibody
  • Clone: SY5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Dog ; Human ; Pig ; Primate
  • Host: Mouse
  • Cancer type: Skin cancer
  • Molecular weight of the target: 66-170 kDa
  • Application: IF ; FACS ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Clone SY5 recognises a protein of 66kDa-170kDa, identified as involucrin. Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. Involucrin expression is altered in pathological conditions such as psoriasis and other benign epidermal hyperplasias. This mAb stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, 66kDa in dog, and a doublet of 105kDa in pig.

    It's epitope maps between codon 421-568 of human involucrin.

    SY5 is useful for studies of changes in involucrin expression in pathological studies.
  • Immunogen: Purified involucrin from human keratinocytes.
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell signaling and signal transduction
  • Myeloma used: P3X63Ag8

  • For Research Use Only

Target Details

  • Target: Involucrin
  • Target molecular weight: 66-170 kDa
  • Target background: In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Application Details

  • Application: IF ; FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Zhong et al. 2016. Cell. 167(1):187-202. PMID: 27662089.
  • •  Morales et al. 2016. Toxicol In Vitro. 36(8):89-96. PMID: 27448499.
  • •  Raj et al. 2016. Int J Cosmet Sci. 39(1):2-10. PMID: 27079667.
  • •  Golinski et al. 2015. Cells Tissues Organs. 200(3-4):227-239. PMID: 26303436.
  • •  Ravindran et al. 2015. Head Neck. 37(7):982-993. PMID: 24700702.
  • •  Creppe et al. 2012. Mol Cell Biol. 32(8):1442-1452. PMID: 22331466.
  • •  White et al. 2010. J Immunol. 185(8):4769-4776. PMID: 20861360.
  • •  Skoog et al. 2009. J Invest Dermatol. 129(1):119-130. PMID: 18633436.
  • •  Hudson et al. 1992. Hybridoma. 11(3):367-379. PMID: 1500072.