ANTIBODIES

Contributor Information
- Name Bill Gullick
- Institute Imperial Cancer Research Fund
Tool Details
- Tool name: Anti-ErbB3 [RTJ1]
- Clone: RTJ1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IP ; WB
- Description: Monoclonal antibody directed against ErbB3 kinase, widely prevalent in GI and breast tumours.
- Immunogen: Synthetic Peptide from C terminus of human c-erbB3
- Immunogen UniProt ID: P21860
- Isotype: IgM
- Research area: Cancer; Cell biology; Cell signaling and signal transduction
- Myeloma used: NS0
- For Research Use Only
Target Details
- Target: ErbB3 (Her3)
- Target background: The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). c-erbB3 is normally found in non-dividing differentiated epithelial cells, neurones and hepatocytes. ErbB3 was found to be kinase impaired, having only 1/1000 the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as an allosteric activator. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2. It has approximately 80% prevalence in a wide variety of tumours of the gastro-intestinal tract, and approximately 20% prevalence in breast cancer. This antibody is specific against c-erbB-3 oncoprotein that is expressed in a variety of human normal, foetal and neoplastic epithelial tissues.
Application Details
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20ðC frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4ðC
References
- • Li et al. 2017. Oncotarget. 8(40):67140-67151. PMID: 28978022.
- • Lattanzio et al. 2016. Oncol Lett. 12(1):453-458. PMID: 27347164.
- • Anagnostou et al. 2010. Cancer Epidemiol Biomarkers Prev. 19(4):982-91. PMID: 20332259.
- • Analytic variability in immunohistochemistry biomarker studies.
- • Kapitanovic et al. 2000. J Cancer Res Clin Oncol. 126(4):205-11. PMID: 10782893.
- • Expression of erbB-3 protein in colorectal adenocarcinoma: correlation with poor survival.
- • Naidu et al. 1998. Br J Cancer. 78(10):1385-90. PMID: 9823984.
- • Expression of c-erbB3 protein in primary breast carcinomas.
- • Srinivasan et al. 1998. J Pathol. 185(3):236-45. PMID: 9771476.
- • Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types.
- • Rajkumar et al. 1996. Clin Mol Pathol. 49(4):M199-202. PMID: 16696074.
- • c-erbB3 protein expression in ovarian cancer.
- • Rajkumar et al. 1993. J Pathol. 170(3):271-8. PMID: 8133400.
- • Expression of the c-erbB-3 protein in gastrointestinal tract tumours determined by monoclonal antibody RTJ1.