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Contributor Information

  • Name David Lane
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-PCNA [PC5]
  • Alternate names: Proliferating Cell Nuclear Antigen; DNA Polymerase Delta Auxiliary Protein; ATLD2
  • Clone: PC5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Insect ; Schizosaccharomyces pombe
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Application: IHC ; WB
  • Description: PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis).
  • Immunogen: Protein A-PCNA fusion obtained from pC2T.
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein
  • Target background: PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis).

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Yu et al. 1995. Anal Cell Pathol. 9(1):45-52. PMID: 7577754.
  • •  Optimization of immunohistochemical staining of proliferating cells in paraffin sections of breast carcinoma using antibodies to proliferating cell nuclear antigen and the Ki-67 antigen.
  • •  Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.
  • •  Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.