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Contributor Information

  • Name Lawrence Banks
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
  • Primary citation Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.

Tool Details

  • Tool name: Anti-p53 [Pab 1801]
  • Clone: Pab 1801
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 53 kDa
  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB
  • Strain: Balb/c
  • Description: p53 is a stress-regulated transcription factor that regulates cell cycle arrest. Mutation of p53 is the most common genetic change so far identified in several major carcinomas. Pab 1801 is a monoclonal antibody for p53. Pab 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species. Pab 1801, recognises an epitope between amino acids 32 and 79 of both wild-type and mutant p53.
  • Immunogen: Purified p53-b-galactosidase fusion proteins
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Genetics
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: p53
  • Target molecular weight: 53 kDa
  • Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.

    Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy.

    Twelve isoforms of p53 have been described. Depending on the location of the epitope, antibodies may detect different isoforms of p53. Mutations to p53 can cause conformational changes that expose different epitopes. Pab 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species. Pab 1801, recognises an epitope between amino acids 32 and 79 of both wild-type and mutant p53.

    Please note that there are some publications that suggest the Pab 1801 clone also cross reacts proteins unrelated to p53 (Wolf et al., 2001. Oncogene. 15;20(11):1307-17. PMID: 11313875; Thomas et al., 2012. PLoS One. 2012; 7(5): e36447 PMID: 22590546).

Application Details

  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.
  • •  Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.
  • •  Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.
  • •  Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.
  • •  Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.
  • •  Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.
  • •  Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.
  • •  Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.
  • •  Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.
  • •  Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.