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Contributor Information

  • Name Ian Hart
  • Institute King's College London
  • Primary citation Schlippe et al. 2000. J Cell Sci. 113:425-437. PMID: 10639330.

Tool Details

  • Tool name: Anti-Integrin alpha V [P2W7] mAb
  • Alternate names: Anti-Integrin antibody; P2W7; anti-alphav; anti-av
  • Clone: P2W7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 150-160 kDa
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Anti-Integrin alpha V [P2W7] is a monoclonal antibody which detects the ectodomain of integrin alpha-V(aV). Cell-matrix interactions are mediated primarily by integrins. Integrins have been implicated in cancer progression and metastasis.

    These integrins are composed of alpha and beta subunits to form a complete signaling molecule.

    Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression.

    Studies have shown P2W7 antibody-induced changes in cell morphology (ZR75-1 cells). The morphological changes noted were transient island formation.
  • Immunogen: Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1
  • Immunogen UniProt ID: P06756
  • Isotype: IgG1
  • Research area: Stem cell biology

  • For Research Use Only

Target Details

  • Target: Integrin alpha V (CD51)
  • Target molecular weight: 150-160 kDa
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin aV associates with integrin b3 to form the osteoclast Fn antigen (vitronectin receptor). The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression. Anti-integrin aV detects the ectodomain of human integrin alpha V/CD51.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.
  • •  Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.
  • •  Murase et al. 2017. J Neurosci Res. 95(12):2500-2510. PMID: 28509387.
  • •  Richards et al. 2011. J Virol. 85(17):8766-8776. PMID: 21715474.
  • •  Misselwitz et al. 2011. Mol Syst Biol. 7(1):474. PMID: 21407211.
  • •  Schlippe et al. 2000. J Cell Sci. 113:425-437. PMID: 10639330.