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Contributor Information

  • Name Mike Horton
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Integrin b3 [MHF4]
  • Clone: MHF4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Rat
  • Host: Mouse
  • Molecular weight of the target: 118/100
  • Application: FACS ; IHC ; FACS ; IHC ; IF ; IP
  • Strain: Balb/c
  • Description: The MHF4 epitope is different to the F11 epitope.
  • Immunogen: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.
  • Isotype: IgG1
  • Research area: Cell biology; Tissue-specific biology; Cell signaling and signal transduction

  • For Research Use Only

Target Details

  • Target: Integrin ?3 chain (CD61)
  • Target molecular weight: 118/100
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets. MHF4 may be used to identify osteoclasts in tissue sections and bone cell suspensions.

Application Details

  • Application: FACS ; IHC ; FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Human Adenovirus Type 37 Uses aV?1 and a3?1 Integrins for Infection of Human Corneal Cells.
  • •  Storm et al. 2016. J Virol. :. PMID: 27974569.
  • •  Ballana et al. 2011. J Immunol. 186(1):464-70. PMID: 21098231.
  • •  ?5 integrin is the major contributor to the aVintegrin-mediated blockade of HIV-1 replication.
  • •  Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
  • •  Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.