ANTIBODIES

Contributor Information
- Name Irene Leigh
- Institute Queen Mary University of London
Tool Details
- Tool name: Anti-Keratin16 [LL025]
- Clone: LL025
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IF ; IP ; WB
- Description: Monoclonal antibody used to detect keratin 16 mutations within epithelial tissues.
- Immunogen: A synthetic peptide corresponding to an amino acid sequence of the carboxyl terminus of human keratin 16.
- Immunogen UniProt ID: P08779
- Isotype: IgG1
- Research area: Cell signaling and signal transduction
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Keratin 16
- Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 16 is expressed in suprabasal keratinocytes of wounded epidermis. Keratin 16 suprabasal expression is found in situations of high cell turnover. Mutations in the gene encoding keratin16 are associated with the genetic skin disorders including pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. LL025 reactivity was completely negative in normal breast tissue and non-invasive breast carcinoma. However, 10% of the invasive carcinomas tested were diffusely or focally positive with this antibody.
Application Details
- Application: IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20ðC frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4ðC
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References
- • Hughes BR et al. 1996. Br. J. Dermatol. 134(2):247-56.PMID: 8746337
- • Wong et al. 2019. Sci Rep. 9(1):18561. PMID: 31811191.
- • Frew et al. 2018. F1000Res. 7:1923. PMID: 31281635.
- • Boink et al. 2018. J Tissue Eng Regen Med. 12(2):e1088-e1097. PMID: 28388010.
- • Ojeh et al. 2017. PLoS One. 12(3):e0174389. PMID: 28350869.
- • Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
- • K15 expression implies lateral differentiation within stratified epithelial basal cells.
- • de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.
- • Keratin expression in discoid lupus erythematosus.
- • Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.
- • Keratin expression in basal cell carcinomas.
- • Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
- • Basal cell-specific and hyperproliferation-related keratins in human breast cancer.