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Contributor Information

  • Name Irene Leigh
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Keratin10 [LH2]
  • Alternate names: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 1, KRT1 Human Entrez Gene ID 3858 Human SwissProt P13645 Human Unigene 99936 Human Gene Symbol KRT1 Human Chromosome Location 17q21.2
  • Clone: LH2
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Molecular weight of the target: 56.5kDa
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: LH2 recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10).
  • Immunogen: extract of skin from psoriasis patient
  • Isotype: IgG1 kappa
  • Research area: Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Keratin 10
  • Target molecular weight: 56.5kDa
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 10 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epthelia. Keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia and is present on all suprabasal keratinocytes. Mutations in keratin 10 cause epidermolytic hyperkeratosis. LH2 does not react with any tumour tissue regardless of histological classification.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Lehman et al. 2015. Am J Pathol. 185(1):240-51. PMID: 25529795.
  • •  Barrott et al. 2011. Proc Natl Acad Sci U S A. 108(31):12752-7. PMID: 21768372.
  • •  Deletion of mouse Porcn blocks Wnt ligand secretion and reveals an ectodermal etiology of human focal dermal hypoplasia/Goltz syndrome.
  • •  Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
  • •  K15 expression implies lateral differentiation within stratified epithelial basal cells.
  • •  Reichelt et al. 1997. J Cell Sci. 110 ( Pt 18):2175-86. PMID: 9378767.
  • •  Out of balance: consequences of a partial keratin 10 knockout.
  • •  Markey et al. 1991. J Invest Dermatol. 97(5):763-70. PMID: 1717607.
  • •  Expression of simple epithelial keratins 8 and 18 in epidermal neoplasia.
  • •  Broekaert et al. 1990. Arch Dermatol Res. 282(6):383-91. PMID: 1701984.
  • •  An investigation of cytokeratin expression in skin epithelial cysts and some uncommon types of cystic tumours using chain-specific antibodies.