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Contributor Information

  • Name Walter Bodmer
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-PLAP [HD 8B6]
  • Clone: HD 8B6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IF ; ELISA ; IHC ; RIA
  • Strain: Balb/c
  • Description: HD 8B6 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes.
  • Immunogen: Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture.
  • Isotype: IgG2a
  • Research area: Cancer; Cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Placental Alkaline Phosphatase (PLAP), Regan and Nagao isoenzymes.
  • Target background: Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Application Details

  • Application: IF ; ELISA ; IHC ; RIA

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Sharp et al. 2010. PLoS One. 5(10):e13130. PMID: 20976190.
  • •  Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.
  • •  Wojtowicz et al. 2007. Cell. 130(6):1134-45. PMID: 17889655.
  • •  A vast repertoire of Dscam binding specificities arises from modular interactions of variable Ig domains.
  • •  Durbin et al. 1988. Int J Cancer Suppl. 2:50-8. PMID: 2450848.
  • •  Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.
  • •  Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.
  • •  Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.