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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Integrin a2 [HAS-4]
  • Alternate names: Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; Human Platelet Alloantigen System 5; Platelet Glycoprotein GPIa; Platelet Antigen Br; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR
  • Clone: HAS-4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP
  • Description: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a2 associates with integrin b1 (CD29) to form the heterodimer integrin a2b1, which is one of the most abundant keratinocyte integrins, is found distributed in the basal layer of epidermis and binds collagen. HAS-4 co-precipitates integrin a2b1 from keratinocytes and inhibits HGF-induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.
  • Isotype: IgG2a
  • Research area: Cell biology; Cancer; Cell signaling and signal transduction; Metabolism
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Integrin a2 (CD49b)
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a2 associates with integrin b1 (CD29) to form the heterodimer integrin a2b1, which is one of the most abundant keratinocyte integrins, is found distributed in the basal layer of epidermis and binds collagen. HAS-4 co-precipitates integrin a2b1 from keratinocytes and inhibits HGF-induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.

Application Details

  • Application: FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Malara et al. 2011. Blood. 117(8):2476-83. PMID: 21131589.
  • •  Megakaryocyte-matrix interaction within bone marrow: new roles for fibronectin and factor XIII-A.
  • •  Owens et al. 2001. Cancer Res. 61(13):5248-54. PMID: 11431366.
  • •  Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.
  • •  Alford et al. 1998. J Cell Sci. 111 ( Pt 4):521-32. PMID: 9443900.
  • •  Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels.
  • •  Tenchini et al. 1993. Cell Adhes Commun. 1(1):55-66. PMID: 7521749.
  • •  Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes.