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Contributor Information

  • Name Peter Goodfellow
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-EGFR [EGFR1]
  • Clone: EGFR1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 175 kDa
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody which binds EGFR1 tyrosine kinase, commonly mutated in a range of cancers.
  • Immunogen: Human epidermoid carcinoma line A431
  • Immunogen UniProt ID: P00533
  • Isotype: IgG2b kappa
  • Research area: Cancer; Cell signaling and signal transduction
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Epidermal Growth Factor Receptor (EGFR, Her1)
  • Target molecular weight: 175 kDa
  • Target background: The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). EGFR activation signals multiple downstream signalling cascade pathways such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways which help in growth and proliferation of cells. Dysregulation of EGFR signalling as a consequence of overexpression, amplification and mutation of the EGFR gene occurs frequently in several types of cancers, including head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, oesophagus, stomach and in squamous cell carcinoma. Tumours become dependent on EGFR signalling to maintain their malignant phenotypes.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Finetti et al. 2015. Endocr Relat Cancer. 22(4):665-78. PMID: 26113609.
  • •  Perillo et al. 2015. Nat Commun. 6:7874. PMID: 26219252.
  • •  Tang et al. 2015. Oncol Rep. 34(1):350-8. PMID: 25955689.
  • •  mPGES-1 in prostate cancer controls stemness and amplifies epidermal growth factor receptor-driven oncogenicity.
  • •  Downregulation of CD9 promotes pancreatic cancer growth and metastasis through upregulation of epidermal growth factor on the cell surface.
  • •  Boersma et al. 2011. J Biol Chem. 286(48):41273-85. PMID: 21979953.
  • •  Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling.
  • •  Jensen et al. 2006. Proc Natl Acad Sci U S A. 103(32):11958-63. PMID: 16877544.
  • •  Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence.
  • •  Gullick et al. 1986. Cancer Res. 46(1):285-92. PMID: 2998607.
  • •  Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas.
  • •  Waterfield et al. 1982. J Cell Biochem. 20(2):149-61. PMID: 6188757.
  • •  A monoclonal antibody to the human epidermal growth factor receptor.