ANTIBODIES

Contributor Information
- Name Walter Bodmer
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-EpCAM [AUA1]
- Clone: AUA1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 40 kDa
- Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
- Strain: Balb/c
- Description: AUA1 cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells.
- Immunogen: LoVo cell line, a colon carcinoma cell line.
- Isotype: IgG1
- Research area: Cancer; Cell biology
- Myeloma used: P3X63Ag8
- For Research Use Only
Target Details
- Target: Epithelial Cell Adhesion Molecule (EpCAM, CD326)
- Target molecular weight: 40 kDa
- Target background: AUA1 cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells.
Application Details
- Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.93mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Hsia et al. 2016. Proc Natl Acad Sci U S A. :. PMID: 27036009.
- • Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers.
- • Screening of carcinoma metastasis by flow cytometry: A study of 238 cases.
- • Acosta et al. 2015. Cytometry B Clin Cytom. :. PMID: 26054018.
- • Yeung et al. 2013. Br J Cancer. 108(10):2106-15. PMID: 23652304.
- • Myofibroblast activation in colorectal cancer lymph node metastases.
- • Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. PMID: 20133591.
- • Cancer stem cells from colorectal cancer-derived cell lines.
- • Gaster et al. 2009. Nat Med. 15(11):1327-32. PMID: 19820717.
- • Matrix-insensitive protein assays push the limits of biosensors in medicine.
- • Ntouroupi et al. 2008. Br J Cancer. 99(5):789-95. PMID: 18682708.
- • Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope.
- • Shaw et al. 2008. Cancer Cell Int. 8:3. PMID: 18348720.
- • Inhibition of androgen-independent prostate cancer cell growth is enhanced by combination therapy targeting Hedgehog and ErbB signalling.
- • Moss et al. 1988. Lung Cancer. 4, 76-78.
- • Epenetos et al. 1982. Lancet. 2(8306):1004-6. PMID: 6127499.
- • Use of two epithelium-specific monoclonal antibodies for diagnosis of malignancy in serous effusions.
- • Epenetos et al. 1982. Br J Cancer. 46(1):1-8. PMID: 7104190.
- • Detection of human cancer in an animal model using radio-labelled tumour-associated monoclonal antibodies.