ANTIBODIES

Contributor Information
- Name Peter Parker
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-Blood group A sugar determinant [9A]
- Clone: 9A
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: FACS ; ELISA ; IHC ; IP
- Description: 9A is suitable for binding to the EGF receptor in A431 cells only.
- Immunogen: A431 cells (human vulval squamous carcinoma cell line)
- Isotype: IgG3
- Research area: Cell biology
- Myeloma used: P3X63Ag8
- For Research Use Only
Target Details
- Target: Blood group A sugar determinant
- Target background: 9A is suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures.
Application Details
- Application: FACS ; ELISA ; IHC ; IP
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
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References
- • Increased a-series gangliosides positively regulate leptin/Ob receptor-mediated signals in hypothalamus of GD3 synthase-deficient mice.
- • Fischer et al. 2015. Front Immunol. 6:211. PMID: 25999949.
- • Gullick et al. 1990. J Pathol. 162(3):275-6. PMID: 2266467.
- • 'EGF receptor' antibody 29.1.1.
- • Parker et al. 1984. J Biol Chem. 259(15):9906-12. PMID: 6086658.
- • Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor.