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Contributor Information

  • Name Susheela Dhut
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-BCRABL [7c6] mAb
  • Clone: 7c6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 210 kDa
  • Application: IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody directed against BCR-ABL oncoprotein.
  • Immunogen: Bcr686 thyroglobulin conjugate
  • Immunogen UniProt ID: Q62199
  • Isotype: IgG2a
  • Research area: Cancer; Cell signaling and signal transduction; Genetics
  • Myeloma used: P3X63Ag8

  • For Research Use Only

Target Details

  • Target: BCRABL
  • Target molecular weight: 210 kDa
  • Target background: The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukaemia (CML) and in a proportion of cases of acute lymphocytic leukaemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9. This results in the production of a chimeric BCR-ABL oncoprotein with constitutively active tyrosine kinase activity. Anti-BCRABL antibody may be useful for studies of the oncogene and have potential diagnostic and prognostic applications, particularly within leukaemia. This antibody is specific for the SSINEEITPRRQS epitope of BCR (residues 686-696), and as such is able to recognise full-length BCR and the p210 form of the BCR-ABL fusion protein.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

Documentation

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References

  • •  Mallery et al. 2017. Cancer Prev Res (Phila). 10(1):76-88. PMID: 27756753.
  • •  Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.
  • •  BCR-ABL and BCR proteins: biochemical characterization and localization.
  • •  Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.
  • •  Identification of two normal bcr gene products in the cytoplasm.
  • •  Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.
  • •  Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.