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Contributor Information

  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-BCRABL [4C12]
  • Clone: 4C12
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Blood cancer
  • Application: IHC ; IF ; RIA
  • Strain: Balb/c
  • Description: The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukemia (CML) and in a proportion of cases of acute lymphocytic leukemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9 and results in the production of a chimaeric BCR-ABL protein with enhanced kinase activity. These antibodies may be useful for studies of the oncogene and have potential diagnostic and prognostic applications.
  • Immunogen: BCR-ABL peptide
  • Isotype: IgG1
  • Research area: Cancer; Cell signaling and signal transduction; Genetics
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: BCRABL
  • Target background: The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukemia (CML) and in a proportion of cases of acute lymphocytic leukemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9 and results in the production of a chimaeric BCR-ABL protein with enhanced kinase activity. These antibodies may be useful for studies of the oncogene and have potential diagnostic and prognostic applications.

Application Details

  • Application: IHC ; IF ; RIA

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.
  • •  BCR-ABL and BCR proteins: biochemical characterization and localization.
  • •  Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.
  • •  Identification of two normal bcr gene products in the cytoplasm.
  • •  Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.
  • •  Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.